Flow cytometric estimation of cell cycle parameters using a monoclonal antibody to bromodeoxyuridine
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منابع مشابه
Flow Cytometric Analysis by Bromodeoxyuridine/DNA Assay of Cell Cycle Perturbation of Methotrexate-treated Mouse LI 210 Leukemia Cells1
The in vitro effects of methotrexate (MTX) on cell cycle progression and DNA synthesis of LI 210 leukemia cells were studied by the bromodeoxyuridine (BrdUrd)/DNA analysis technique. Low dose (10~8 M) MTX which slightly inhibits clonal replication of the cells, delays progress across the S phase, and treatment for 24 h results in a slight increase of the S-phase population. Much higher doses (I...
متن کاملFlow cytometric analysis by bromodeoxyuridine/DNA assay of cell cycle perturbation of methotrexate-treated mouse L1210 leukemia cells.
The in vitro effects of methotrexate (MTX) on cell cycle progression and DNA synthesis of L1210 leukemia cells were studied by the bromodeoxyuridine (BrdUrd)/DNA analysis technique. Low dose (10(-8) M) MTX, which slightly inhibits clonal replication of the cells, delays progress across the S phase, and treatment for 24 h results in a slight increase of the S-phase population. Much higher doses ...
متن کاملBromodeoxyuridine in tumors and chromosomes detected with a monoclonal antibody.
Using a monoclonal antibody to bromodeoxyuridine (BUdR) and immunohistochemistry, we measured the incorporation of this thymidine analogue into the DNA of human normal and malignant cells exposed in vivo. BUdR given as a constant intravenous infusion for 12 or 24 h daily for up to 13 d resulted in a steady-state plasma level of 10(-6) M during the infusion. We demonstrated extensive incorporati...
متن کاملCorrection: a method to estimate cell cycle time and growth fraction using bromodeoxyuridine-flow cytometry data from a single sample
References 1. Eidukevicius R, Characiejus D, Janavicius R, Kazlauskaite N, Pasukoniene V, Mauricas M, Den Otter W: A method to estimate cell cycle time and growth fraction using bromodeoxyuridineflow cytometry data from a single sample. BMC Cancer 2005, 5:122. 2. Johansson MC, Baldetorp B, Bendahl PO, Johansson R, Oredsson SM: An improved mathematical method to estimate DNA synthesis time of br...
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capture ELISA system was developed for diagnosis of visceral leishmaniasis (VL) using a monoclonal antibody raised against an antigen previously detected in the urine of VL patients. Urine samples from confirmed VL cases from Yemen, Nepal, Spain, Sudan and Brazil were tested in the capture ELISA in comparison with urine samples from endemic and non-endemic areas along with urine samples from pa...
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ژورنال
عنوان ژورنال: Cytometry
سال: 1986
ISSN: 0196-4763,1097-0320
DOI: 10.1002/cyto.990070415